1. Set the power switch to "on" and turn the brightness to 7
   
2. Switch the condenser at the middle position
   
3. Let 4x objective into position and turn the coarse focus knob to move the stage uppermost (watch the distance between the 4x objective with specimen).
   
4. Examine the specimen with naked eye, then place the specimen on the stage and use stage motion control knobs to move the specimen into the optical path. Adjust the interpupillary distance so that the left and right viewfields become one.
   
5. Focus on the specimen with a 4x objective in the first place with the coarse focus knob (the direction which moves the stage from up to down).
   
6. Then, change the magnification to 10x and 40x objectives by rotating the revolving nosepiece. If focusing is attempted with 40x objective, you must use fine focus knob rather than coarse one.

Note: Never let the 100x objective slip through the specimen

Before you return the microscope

1. Collect the specimen slides
2. Turn the brightness to 1
3. Set the power switch to "off"
4. Turn 4x objective into the optical path
5. Move the stage to the lowest with coarse focus knob